Abstract |
The expression of the T4 denV gene, which previously had been cloned in plasmid constructs downstream of the bacteriophage lambda hybrid promoter-operator oLpR, was analyzed under a variety of growth parameters. Expression of the denV gene product, endonuclease V, was confirmed in DNA repair-deficient Escherichia coli (uvrA recA) by Western blot analyses and by enhancements of resistance to UV irradiation.
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Authors | A Recinos 3rd, M L Augustine, K M Higgins, R S Lloyd |
Journal | Journal of bacteriology
(J Bacteriol)
Vol. 168
Issue 2
Pg. 1014-8
(Nov 1986)
ISSN: 0021-9193 [Print] United States |
PMID | 3536845
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA, Recombinant
- Endodeoxyribonucleases
- Deoxyribonuclease (Pyrimidine Dimer)
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Topics |
- Cloning, Molecular
- DNA, Recombinant
- Deoxyribonuclease (Pyrimidine Dimer)
- Endodeoxyribonucleases
(biosynthesis, genetics)
- Escherichia coli
(enzymology, genetics, radiation effects)
- Genes
- Genes, Viral
- Plasmids
- T-Phages
(enzymology, genetics)
- Ultraviolet Rays
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