HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Expression of the bacteriophage T4 denV structural gene in Escherichia coli.

Abstract
The expression of the T4 denV gene, which previously had been cloned in plasmid constructs downstream of the bacteriophage lambda hybrid promoter-operator oLpR, was analyzed under a variety of growth parameters. Expression of the denV gene product, endonuclease V, was confirmed in DNA repair-deficient Escherichia coli (uvrA recA) by Western blot analyses and by enhancements of resistance to UV irradiation.
AuthorsA Recinos 3rd, M L Augustine, K M Higgins, R S Lloyd
JournalJournal of bacteriology (J Bacteriol) Vol. 168 Issue 2 Pg. 1014-8 (Nov 1986) ISSN: 0021-9193 [Print] United States
PMID3536845 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • DNA, Recombinant
  • Endodeoxyribonucleases
  • Deoxyribonuclease (Pyrimidine Dimer)
Topics
  • Cloning, Molecular
  • DNA, Recombinant
  • Deoxyribonuclease (Pyrimidine Dimer)
  • Endodeoxyribonucleases (biosynthesis, genetics)
  • Escherichia coli (enzymology, genetics, radiation effects)
  • Genes
  • Genes, Viral
  • Plasmids
  • T-Phages (enzymology, genetics)
  • Ultraviolet Rays

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: