Abstract |
A commercial antibody capture enzyme immunoassay (Rubenz M) was compared to two commercial indirect enzyme immunoassays (Enzygnost IgM, Rubazyme-M) for the detection of rubella-specific IgM. Five hundred and fifty-two sera collected between the day of onset and 272 days after the onset of the exanthem of primary rubella were tested. Rubenz M was more sensitive early and late after the onset of the exanthem than the two indirect ELISAs. Rubenz M also appeared more sensitive when 240 sera were examined from patients with possible rubella in pregnancy, reinfection in pregnancy, suspected intrauterine infection, and recent vaccination. However, 5.5% of 968 pregnant women with no history of rubellalike symptoms or recent vaccination, the majority with elevated HAI titers, gave a low-positive or borderline result with Rubenz M. None of these women delivered a congenitally infected child. Therefore, borderline and low-positive results must be interpreted with caution, as for any assay for rubella-specific IgM.
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Authors | G Enders, F Knotek |
Journal | Journal of medical virology
(J Med Virol)
Vol. 19
Issue 4
Pg. 377-86
(Aug 1986)
ISSN: 0146-6615 [Print] United States |
PMID | 3528389
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antibodies, Viral
- Immunoglobulin M
- Reagent Kits, Diagnostic
- Rheumatoid Factor
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Topics |
- Antibodies, Viral
(analysis)
- Child
- Enzyme-Linked Immunosorbent Assay
- False Positive Reactions
- Female
- Humans
- Immunoenzyme Techniques
- Immunoglobulin M
(analysis)
- Pregnancy
- Pregnancy Complications, Infectious
(diagnosis)
- Reagent Kits, Diagnostic
- Rheumatoid Factor
- Rubella
(diagnosis)
- Rubella virus
(immunology)
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