Probiotic characterization of Lactiplantibacillus plantarum HOM3204 and its restoration effect on antibiotic-induced dysbiosis in mice.

Abstract

The purpose of this study was to evaluate the probiotic characteristics of Lactiplantibacillus plantarum HOM3204 isolated from homemade pickled cabbage and to examine its restoration effect on antibiotic-induced dysbiosis in mice. Lact. plantarum HOM3204 tolerated simulated gastric and intestinal juices with a 99·38% survival rate. It also showed strong adhesion ability (3·45%) to Caco-2 cells and excellent antimicrobial activity against foodborne pathogens in vitro. For safety (antibiotic susceptibility) of this strain, it was susceptible to all the tested seven antibiotics. Lact. plantarum HOM3204 had good stability during storage, especially in cold and frozen conditions. Furthermore, Lact. plantarum HOM3204 significantly restored the gut microbiota composition by increasing the abundance of Lactobacilli and Bifidobacteria and decreasing Enterococci, and improved antioxidative function by raising the concentrations of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in serum of antibiotic-induced dysbiosis in mice. These results suggest that Lact. plantarum HOM3204 could be a potential probiotic as a functional food ingredient.

• Authors: S Zhang, T Wang, D Zhang, X Wang, Z Zhang, C Lim, S Lee
• Journal: Letters in applied microbiology (Lett Appl Microbiol) Vol. 74 Issue 6 Pg. 949-958 (Jun 2022) ISSN: 1472-765X [Electronic] England
• PMID: 35231139 (Publication Type: Journal Article)
• Copyright: © 2022 The Authors. Letters in Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.

Chemical References

• Anti-Bacterial Agents

Topics

• Animals
• Anti-Bacterial Agents
• Caco-2 Cells
• Dysbiosis (chemically induced)
• Humans
• Lactobacillaceae
• Lactobacillus plantarum
• Mice
• Probiotics (pharmacology)