Intraoperative near-infrared fluorescence imaging can identify pelvic nerves in patients with cervical cancer in real time during radical hysterectomy.

Abstract

PURPOSE:

Radical hysterectomy combined with pelvic lymphadenectomy is the standard treatment for early-stage cervical cancer, but unrecognized pelvic nerves are vulnerable to irreversible damage during surgery. This early clinical trial investigated the feasibility and safety of intraoperative near-infrared (NIR) fluorescence imaging (NIR-FI) with indocyanine green (ICG) for identifying pelvic nerves during radical hysterectomy for cervical cancer.

METHODS:

Sixty-six adults with cervical cancer were enrolled in this prospective, open-label, single-arm, single-center clinical trial. NIR-FI was performed in vivo to identify genitofemoral (GN), obturator (ON), and hypogastric (HN) nerves intraoperatively. The primary endpoint was the presence of fluorescence in pelvic nerves. Secondary endpoints were the ICG distribution in a nerve specimen and potential underlying causes of fluorescence emission in pelvic nerves.

RESULTS:

In total, 63 patients were analyzed. The ON was visualized bilaterally in 100% (63/63) of patients, with a mean fluorescence signal-to-background ratio (SBR) of 5.3±2.1. The GN was identified bilaterally in 93.7% (59/63) of patients and unilaterally in the remaining 4 patients, with a mean SBR of 4.1±1.9. The HN was identified bilaterally in 81.0% (51/63) of patients and unilaterally in 7.9% (5/63) of patients, with a mean SBR of 3.5±1.3. ICG fluorescence was detected in frozen sections of a nerve specimen, and was mainly distributed in axons. No ICG-related complications were observed.

CONCLUSION:

This early clinical trial demonstrated the feasibility and safety of NIR-FI to visualize pelvic nerves intraoperatively. Thus, NIR-FI may help surgeons adjust surgical decision-making, avoid nerve damage, and improve surgical outcomes.

TRIAL REGISTRATION:

ClinicalTrials.gov NCT04224467.

• Authors: Kunshan He, Pengfei Li, Zeyu Zhang, Jiaqi Liu, Pan Liu, Shipeng Gong, Chongwei Chi, Ping Liu, Chunlin Chen, Jie Tian
• Journal: European journal of nuclear medicine and molecular imaging (Eur J Nucl Med Mol Imaging) Vol. 49 Issue 8 Pg. 2929-2937 (07 2022) ISSN: 1619-7089 [Electronic] Germany
• PMID: 35230489 (Publication Type: Clinical Trial, Journal Article)
• Copyright: © 2022. The Author(s).

Chemical References

• Indocyanine Green

Topics

• Adult
• Female
• Humans
• Hysterectomy (adverse effects, methods)
• Indocyanine Green
• Optical Imaging (methods)
• Prospective Studies
• Sentinel Lymph Node Biopsy (methods)
• Spectroscopy, Near-Infrared (methods)
• Uterine Cervical Neoplasms (diagnostic imaging, surgery)