Urocortin-positive nerve fibres and cells are present in the human choroid.

Abstract

BACKGROUND:

Choroidal vascular regulation is mediated by the autonomic nervous system in order to gain proper blood flow control. While the mechanisms behind this control are unknown, neuroregulatory peptides are involved in this process. To better understand choroidal function, we investigate the presence of urocortin-1 (UCN), a neuroregulatory peptide with vascular effects, in the human choroid and its possible intrinsic and extrinsic origin.

METHODS:

Human choroid and eye-related cranial ganglia (superior cervical ganglion- SCG, ciliary ganglion-CIL, pterygopalatine ganglion-PPG, trigeminal ganglion-TRI) were prepared for immunohistochemistry against UCN, protein-gene product 9.5 (PGP9.5), substance P (SP), tyrosine hydroxylase (TH) and vesicular acetylcholine transporter (VAChT). For documentation, confocal laser scanning microscopy was used.

RESULTS:

In choroidal stroma, UCN-immunoreactivity was present in nerve fibres, small cells and intrinsic choroidal neurons (ICN). Some UCN+ nerve fibres colocalised for VAChT, while others were VAChT. A similar situation was found with SP: some UCN+ nerve fibres showed colocalisation for SP, while others lacked SP. Colocalisation for UCN and TH was not observed. In eye-related cranial ganglia, only few cells in the SCG, PPG and TRI were UCN+, while many cells of the CIL displayed weak UCN immunoreactivity.

CONCLUSION:

UCN is part of the choroidal innervation. UCN+/VAChT+ fibres could derive from the few cells of the PPG or cells of the CIL, if these indeed supply the choroid. UCN+/SP+ fibres might originate from ICN, or the few UCN+ cells detected in the TRI. Further studies are necessary to establish UCN function in the choroid and its implication for choroidal autonomic control.

• Authors: Alexandra Kaser-Eichberger, Christian Platzl, Heidi Wolfmeier, Andrea Trost, Anja Horn, Miriam Barnerssoi, Clemens Strohmaier, Falk Schroedl
• Journal: The British journal of ophthalmology (Br J Ophthalmol) Vol. 107 Issue 10 Pg. 1575-1582 (10 2023) ISSN: 1468-2079 [Electronic] England
• PMID: 35217514 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © Author(s) (or their employer(s)) 2023. No commercial re-use. See rights and permissions. Published by BMJ.

Chemical References

• Urocortins
• Substance P

Topics

• Humans
• Urocortins (analysis)
• Nerve Fibers
• Choroid
• Neurons (chemistry, physiology)
• Immunohistochemistry
• Substance P