[Diagnostic preparations based on monoclonal antibodies].

Abstract

The specific activity of diagnostic preparations based on monoclonal IgM MAK-14-7 interacting with the antigens of the Venezuela equine encephalomyelitis virus, monoclonal IgG KAMA-51 interacting with the antigens of the forest spring encephalitis virus and monoclonal IgA OKA and IgM OKA binding to the antigens of the vaccine virus was studied by the enzymatic immunological and immunofluorescent tests. Both the ascitic preparations of the antibodies and the fluorescein isothiocyanate-conjugated reagents such as MAK-FITC, KAMA-FITC and OKA-FITC were characterized by high specificity and activity. The specific sensitivity and activity of the monoclonal immunoglobulins was completely maintained in the mixed and combined preparations.

• Authors: S Ia Gaĭdamovich, A S Novokhatskiĭ, D N Nosik, I N Bespalova, N E Litvinovich
• Journal: Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology (Antibiot Med Biotekhnol) Vol. 31 Issue 4 Pg. 268-73 (Apr 1986) ISSN: 0233-7525 [Print] Russia (Federation)
• Vernacular Title: Diagnosticheskie preparaty na osnove monoklonal'nykh antitel.
• PMID: 3521470 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Viral Vaccines

Topics

• Animals
• Antibodies, Monoclonal (immunology, isolation & purification)
• Cell Line
• Chlorocebus aethiops
• Cricetinae
• Encephalitis Virus, Venezuelan Equine (immunology)
• Encephalitis Viruses, Tick-Borne (immunology)
• Fluorescent Antibody Technique
• HeLa Cells (microbiology)
• Humans
• Immunoenzyme Techniques
• Mesocricetus
• Swine
• Viral Vaccines (immunology)
• Virus Cultivation