Abstract |
By means of indirect immunofluorescence (IFF) tests with rabbit antiserum to Forssman (F) glycolipid, expression of F antigen was investigated on 16 rat tumor cell lines; 14 chemical carcinogen-induced tumors, one yolk sac-derived (AT-1) tumor and another spontaneous tumor. Four lines of the carcinogen-induced tumors gave weakly positive reactions in the IIF tests and the AT-1 cells a strongly positive reaction. Flow fluorocytometric analyses on the AT-1 cell line revealed that some of the major and the vast majority of the minor population of the AT-1 express F antigen. Specificity of the F antigen demonstrated on AT-1 cells was determined by absorption experiments, in which absorption of the F antiserum with guinea pig kidney sediment, sheep red blood cells and F- liposome but not bovine red blood cells or G- liposome abolished the reaction. Results of this study together with those of our previous studies demonstrated that F antigen appears as a result of malignant transformation of F negative rat cells induced by chemical carcinogens, apparently "distorted differentiation" and the viral oncogenes.
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Authors | Y Shigyo, T Mori, K Kano |
Journal | Immunological investigations
(Immunol Invest)
Vol. 15
Issue 1
Pg. 25-33
(Mar 1986)
ISSN: 0882-0139 [Print] England |
PMID | 3519448
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antigens, Heterophile
- Antigens, Surface
- Forssman Antigen
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Topics |
- Animals
- Antibody Specificity
- Antigens, Heterophile
(analysis)
- Antigens, Surface
(analysis)
- Cell Line
- Flow Cytometry
(methods)
- Fluorescent Antibody Technique
- Forssman Antigen
(analysis, immunology)
- Mesonephroma
(immunology)
- Neoplasms, Experimental
(immunology)
- Rats
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