It is reported that oxidative stress homeostasis was involved in PM2.5-induced foam cell formation and progression of
atherosclerosis, but the exact molecular mechanism is still unclear.
Melatonin is an effective
antioxidant that could reverse the cardiopulmonary injury. The main purpose of this study is to investigate the latent mechanism of PM2.5-triggered
atherosclerosis development and the protective role of
melatonin administration. Vascular Doppler ultrasound showed that PM2.5 exposure reduced aortic elasticity in
ApoE-/- mice. Meanwhile, blood biochemical and pathological analysis demonstrated that PM2.5 exposure caused
dyslipidemia, elicited oxidative damage of aorta and was accompanied by an increase in
atherosclerotic plaque area; while the
melatonin administration could effectively alleviate PM2.5-induced macrophage M1 polarization and
atherosclerosis in mice. Further investigation verified that
NADPH oxidase 2 (NOX2) and mitochondria are two prominent sources of PM2.5-induced ROS production in vascular macrophages. Whereas, the combined use of two ROS-specific inhibitors and adopted with
melatonin markedly rescued PM2.5-triggered macrophage M1 polarization and foam cell formation by inhibiting NOX2-mediated crosstalk of Keap1/Nrf2/NF-κB and TLR4/
TRAF6/NF-κB signaling pathways. Our results demonstrated that NOX2-mediated oxidative stress homeostasis is critical for PM2.5-induced
atherosclerosis and
melatonin might be a potential treatment for air pollution-related
cardiovascular diseases.