Abstract |
Eight choriocarcinoma cell lines (Two SCH and IMa, were established from non-gestational choriocarcinoma and the other six BeWo, JaR, JEG-3, GCH-1, NUC-1, and HCCM-5, were from gestational one) were investigated for HLA-Class I and Class II antigen expression by the immunofluorescence method using rabbit anti HLA-ABC serum, and by a radioimmunoassay inhibition best using monoclonal antibodies (W6/32: anti HLA-ABC, 7B6: anti HLA-Class II, and D1-4-10: anti HLA-DR). With respect to HLA- Class I antigen expression, only non-gestational SCH (gastric origin) showed a clear positive finding. The other non-gestational IMa (ovarian origin) was negative. Cell lines from gestational choriocarcinoma showed a slightly positive finding (negative by immunofluorescence but slightly positive by inhibition test), except for JaR which was negative. All cell lines examined in the present study were negative for HLA- Class II antigen. The present results show that the majority of the gestational choriocarcinoma cell lines may express very slight HLA- Class I antigens.
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Authors | K Yamashita, T Nakamura, T Shimizu, H Ikeda, M Katagiri |
Journal | Nihon Sanka Fujinka Gakkai zasshi
(Nihon Sanka Fujinka Gakkai Zasshi)
Vol. 38
Issue 1
Pg. 17-22
(Jan 1986)
ISSN: 0300-9165 [Print] Japan |
PMID | 3512743
(Publication Type: English Abstract, Journal Article)
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Chemical References |
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Topics |
- Cell Line
- Choriocarcinoma
(immunology)
- Female
- Fluorescent Antibody Technique
- HLA Antigens
(analysis, classification)
- Humans
- Pregnancy
- Radioimmunoassay
- Uterine Neoplasms
(immunology)
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