Measurement of human and mouse anti-tetanus antibodies and isotype analysis by ELISA.

Abstract

A rapid and sensitive enzyme immunoassay (ELISA) was developed for the quantitation of anti-tetanus antibodies. This technique was used to measure antibody levels in the plasma of immunized donors, in human anti-tetanus IgG preparations and in human and mouse hybridomas producing monoclonal antibodies to tetanus toxoid. The assay was capable of detecting antibody levels as low as 5 X 10(-4) IU/ml. By inclusion of an extra step involving antibodies to mouse Ig isotypes, a sandwich enzyme immunoassay (SEI) was developed which permitted determination of the Ig isotype of mouse anti-tetanus antibodies including tetanus-specific mouse monoclonal antibodies. SEI confirmed Protein A-Sepharose fractionation of mouse ascites fluid containing anti-tetanus antibody. The tetanus toxoid-coated plates have a shelf life of at least 1 year.

• Authors: Z Farzad, K James, D B McClelland
• Journal: Journal of immunological methods (J Immunol Methods) Vol. 87 Issue 1 Pg. 119-25 (Feb 27 1986) ISSN: 0022-1759 [Print] Netherlands
• PMID: 3512719 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Immunoglobulin Allotypes
• Tetanus Antitoxin
• Tetanus Toxoid

Topics

• Animals
• Antibodies, Monoclonal
• Enzyme-Linked Immunosorbent Assay (standards)
• Humans
• Immunoenzyme Techniques
• Immunoglobulin Allotypes (analysis)
• Mice
• Tetanus Antitoxin (analysis)
• Tetanus Toxoid (immunology)