Simple determination of forphenicinol in human plasma and erythrocytes by HPLC with native fluorescence detection.

Abstract

A high performance liquid chromatographic method is described for monitoring forphenicinol, a possible therapeutic drug for cancer and muscular dystrophy, in human plasma and erythrocytes. Forphenicinol in the deproteinized samples was separated from interfering biogenic substances on an aminopropyl-bonded silica (Unicil NH2) column within 10 minutes with isocratic elution, and determined with fluorescence detection. The detection limits for forphenicinol in plasma and erythrocytes are 65 pmol (12.8 ng)/ml and 160 pmol (31.5 ng)/ml, respectively, corresponding to 2 pmol each in a 100 microliters injection volume. The method is very simple, and sensitive enough to permit the quantification of forphenicinol in the blood samples from man dosed with forphenicinol.

• Authors: M Kai, K Tamura, M Ohno, Y Ohkura
• Journal: Biomedical chromatography : BMC (Biomed Chromatogr) Vol. 1 Issue 4 Pg. 143-6 (Aug 1986) ISSN: 0269-3879 [Print] England
• PMID: 3506826 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• forphenicinol
• Glycine

Topics

• Adjuvants, Immunologic (analysis, blood)
• Chromatography, High Pressure Liquid
• Erythrocytes (analysis)
• Glycine (analogs & derivatives, analysis, blood)
• Humans
• Spectrometry, Fluorescence