Measurement of Donath-Landsteiner antibody-producing cells in idiopathic nonsyphilitic paroxysmal cold hemoglobinuria (PCH) in children.

Abstract

A 2-yr-old girl with a nonsyphilitic type of paroxysmal cold hemoglobinuria (PCH) is reported. We applied the plaque-forming cell (PFC) assay to the measurement of Donath-Landsteiner (DL) antibody-producing cells in the patient. Some of the circulating B lymphocytes formed plaques in our assay system. Initially the number of PFC was 1442 +/- 225/10(6) lymphocytes and DL titer was 1:16. Thereafter the PFC decreased in number as the DL titer decreased. Eight weeks later circulating PFC were not found but the DL titer was still 1:1 positive. The PFC assay appears to be useful as a new method for obtaining rapid imformation as to the disease status of PCH.

• Authors: Y Miyagawa, S Yamada, A Komiyama, T Akabane
• Journal: Blood (Blood) Vol. 52 Issue 1 Pg. 97-101 (Jul 1978) ISSN: 0006-4971 [Print] United States
• PMID: 350317 (Publication Type: Case Reports, Journal Article)

Topics

• Antibody-Producing Cells (immunology)
• B-Lymphocytes (immunology)
• Child, Preschool
• Female
• Hemoglobinuria, Paroxysmal (immunology)
• Hemolytic Plaque Technique
• Humans