Immunobeads test (antibody coated polyacrylamide beads) in the immunological characterization of lymphoproliferative disorders.

Abstract

Three groups of patients with immunoproliferative disorders (15 multiple myeloma, 11 non-Hodgkin's lymphoma, 21 chronic lymphocytic leukemia) were studied by immunological characterization and compared to a group of 20 normal subjects (controls) using anti-immunoglobulin coated polyacrylamide beads (T-B Quantigen test, QT), erythrocyte rosettes (ER), surface immunoglobulin (SIg), and monoclonal antibodies for T and B cells (OKT3; OKT11; OKT8; OKT4; IaDR); null cells (NC) and double marker (DM) cells were also considered. The values for normal subjects for T-B, NC and DM cells were comparable. Results for the patient groups strikingly differed. There were progressively larger differences between the T and B percentages obtained with different techniques. The largest differences were seen in patients with chronic lymphocytic leukemia and the smallest in multiple myeloma patients; values were intermediate in non-Hodgkin lymphoma. The different findings were related to the number of DM cells (ER+, SIg+ QT+) and the different tests used. The importance of these findings in the diagnostic approach to lymphoproliferative disorders is discussed.

• Authors: T Chisesi, M Vespignani, G Capnist
• Journal: The International journal of biological markers (Int J Biol Markers) 1987 Sep-Dec Vol. 2 Issue 3 Pg. 173-6 ISSN: 0393-6155 [Print] United States
• PMID: 3502472 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Acrylic Resins
• Antibodies, Monoclonal
• Immunoglobulins
• polyacrylamide

Topics

• Acrylic Resins
• Antibodies, Monoclonal
• B-Lymphocytes (analysis, classification)
• Humans
• Immunoglobulins
• Lymphocytes, Null (analysis)
• Lymphoproliferative Disorders (blood, diagnosis)
• Microspheres
• Phenotype
• Rosette Formation
• T-Lymphocytes (analysis, classification)