Knockdown of lncRNA CCAT1 Inhibits the Progression of Colorectal Cancer via hsa-miR-4679 Mediating the Downregulation of GNG10.

Abstract

Great concerns have raised crucial roles of long noncoding RNAs (lncRNAs) on colorectal cancer progression due to the increasing number of studies in cancer development. Previous studies reveal that lncRNA CCAT1 plays an important role in the progression of a variety of cancers. However, the role of lncRNA CCAT1 in colorectal cancer is still unclear. In this study, we found that in both colorectal tissues and cell lines the level of lncRNA CCAT1 was increased. Downregulation of lncRNA CCAT1 inhibited the proliferation, migration, and invasion of colorectal cell lines and promoted apoptosis. We then found that hsa-miR-4679 could bind to lncRNA CCAT1 directly, and with further functional analyses, we confirmed that lncRNA CCAT1 sponged hsa-miR-4679 to promote the progression of colorectal cancer. Next, we found that hsa-miR-4679 was directly bound to 3'UTR of GNG10 (guanine nucleotide-binding protein, gamma 10). GNG10 overexpression promoted the progression of colorectal cancer, and this phenotype could be reversed by miR-4679 mimics. At last, we knocked down CCAT1 in vivo and found that sh-CCAT1 reduced the tumor size and the number of proliferating cells. In summary, our findings revealed that lncRNA CCAT1 facilitated colorectal cancer progression via the hsa-miR-4679/GNG10 axis and provided new potential therapeutic targets for colorectal cancer.

• Authors: Ning Wang, Jun Li, Ju He, Yong-Guang Jing, Wei-Dong Zhao, Wen-Jin Yu, Jing Wang
• Journal: Journal of immunology research (J Immunol Res) Vol. 2021 Pg. 8930813 ( 2021) ISSN: 2314-7156 [Electronic] Egypt
• PMID: 35005034 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Ning Wang et al.

Chemical References

• GTP-Binding Protein gamma Subunits
• MicroRNAs
• RNA, Long Noncoding
• RNA, Small Interfering
• MIRN4679-1 microRNA, Human
• GNG10 protein, human

Topics

• Animals
• Humans
• Mice
• Carcinogenesis
• Colon (metabolism, pathology)
• Colorectal Neoplasms (genetics, metabolism)
• Down-Regulation
• Gene Expression Regulation, Neoplastic
• GTP-Binding Protein gamma Subunits (genetics, metabolism)
• HT29 Cells
• Mice, Inbred BALB C
• Mice, Nude
• MicroRNAs (genetics)
• RNA, Long Noncoding (genetics)
• RNA, Small Interfering (genetics)
• Tumor Burden
• Xenograft Model Antitumor Assays