Abstract |
The efficient capture and high-activity release of circulating tumor cells (CTCs) remain a challenge for the microfluidic chip. We have fabricated a dual-mode gelatin- polydimethylsiloxane (PDMS) microfluidic chip with a sawtooth columnar microstructure of channels that can capture the efficiency of this substrate up to 95%. Increasing the gelatin temperature to physiologic temperature (37 °C) with a baking table facilitates the overall release of CTCs. With melting of the gelatin substrate, the cells will enter into the PDMS corresponding channel; however, the released cells will be wrapped by a layer of gelatin, which may affect further proliferation of the cells. Another way to release CTCs is to use a matrix metalloproteinase-9 (MMP-9) enzyme to dissolve the gelatin and release the tumor cells with high cellular viability. This dual-mode microfluidic chip has been applied successfully to capturing and releasing CTCs, thus serving as a powerful clinical tool that facilitates the subsequent bioanalysis of CTCs for clinical and biological research.
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Authors | Rui Li, Heng Cui, Zitong Zhang, Lingling Zhang, Yuan Wang, Yongchang Wei, Fuling Zhou, Wei Liu, Wenfei Dong, Xingzhong Zhao, Shishang Guo |
Journal | ACS applied bio materials
(ACS Appl Bio Mater)
Vol. 1
Issue 3
Pg. 910-916
(Sep 17 2018)
ISSN: 2576-6422 [Electronic] United States |
PMID | 34996185
(Publication Type: Journal Article)
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