Graphene oxide-graphene Van der Waals heterostructure transistor biosensor for SARS-CoV-2 protein detection.

Abstract

The current outbreaking of the coronavirus SARS-CoV-2 pandemic threatens global health and has caused serious concern. Currently there is no specific drug against SARS-CoV-2, therefore, a fast and accurate diagnosis method is an urgent need for the diagnosis, timely treatment and infection control of COVID-19 pandemic. In this work, we developed a field effect transistor (FET) biosensor based on graphene oxide-graphene (GO/Gr) van der Waals heterostructure for selective and ultrasensitive SARS-CoV-2 proteins detection. The GO/Gr van der Waals heterostructure was in-situ formed in the microfluidic channel through π-π stacking. The developed biosensor is capable of SARS-CoV-2 proteins detection within 20 min in the large dynamic range from 10 fg/mL to 100 pg/mL with the limit of detection of as low as ∼8 fg/mL, which shows ∼3 × sensitivity enhancement compared with Gr-FET biosensor. The performance enhancement mechanism was studied based on the transistor-based biosensing theory and experimental results, which is mainly attributed to the enhanced SARS-CoV-2 capture antibody immobilization density due to the introduction of the GO layer on the graphene surface. The spiked SARS-CoV-2 protein samples in throat swab buffer solution were tested to confirm the practical application of the biosensor for SARS-CoV-2 proteins detection. The results indicated that the developed GO/Gr van der Waals heterostructure FET biosensor has strong selectivity and high sensitivity, providing a potential method for SARS-CoV-2 fast and accurate detection.

• Authors: Jianwei Gao, Chunhua Wang, Yujin Chu, Yingkuan Han, Yakun Gao, Yanhao Wang, Chao Wang, Hong Liu, Lin Han, Yu Zhang
• Journal: Talanta (Talanta) Vol. 240 Pg. 123197 (Apr 01 2022) ISSN: 1873-3573 [Electronic] Netherlands
• PMID: 34996016 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• graphene oxide
• Graphite

Topics

• Biosensing Techniques
• COVID-19
• Graphite
• Humans
• Pandemics
• SARS-CoV-2