Determinative Structural Features Identified With Probe-based Confocal Endomicroscopy for the Accurate Diagnosis of Gallbladder Malignancy.

Abstract

BACKGROUND:

Probe-based confocal laser endomicroscopy (pCLE) can visualize microscopic structures at high resolution but has not yet yielded definitive diagnostic features of gallbladder malignancy, as opposed to benign changes.

PATIENTS AND METHODS:

A total of 73 patients had their gallbladder evaluated with pCLE performed on resected benign and malignant gallbladder surgical specimens, which were sprayed with fluorescein. Malignant and benign features of pCLE findings were identified on the basis of Miami and Paris Classifications. Standard histopathological diagnoses and individual patient pCLE findings of gallbladder lesions were correlated.

RESULTS:

Of the 73 consecutive patients that had their gallbladder evaluated ex vivo with pCLE, 11 were identified with gallbladder malignancy. pCLE identified features of gallbladders examined ex vivo, including the presence of thick dark bands and dark clumps, which together correlated with histopathologically-determined biliary malignancy at 100% sensitivity. Thick white bands and visualized epithelium, also identified with pCLE, together correlated with histopathologically-determined malignancy at 100% specificity.

CONCLUSION:

pCLE can be used for real-time differentiation of cancerous/non-cancerous regions in the gallbladder using the diagnostic criteria identified in the present study.

• Authors: Tomotake Koizumi, Takeshi Aoki, Toshiko Yamochi, Yoshihiko Tashiro, Tatsuya Yamazaki, Tomokazu Kusano, Kazuhiro Matsuda, Yusuke Wada, Hideki Shibata, Kazuhiko Saito, Koji Nogaki, Tomoki Hakozaki, Kodai Tomioka, Takahito Hirai, Akira Fujimori, Doaa A Mansour, Ahmed Elewa Abbas Gahin, Robert M Hoffman
• Journal: Anticancer research (Anticancer Res) Vol. 42 Issue 1 Pg. 67-73 (Jan 2022) ISSN: 1791-7530 [Electronic] Greece
• PMID: 34969710 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Topics

• Endoscopy (methods)
• Female
• Gallbladder Neoplasms (diagnosis)
• Humans
• Male
• Microscopy, Confocal (methods)