The effect of silyl substituted methotrexate in the treatment of experimental allergic encephalomyelitis in rats mediated by T-line cells.

Abstract

The effect of methotrexate and of silyl-methotrexate were compared in the treatment of experimental allergic encephalomyelitis (EAE) mediated by T-line lymphocytes. It was demonstrated that, during the first three days after cell transfer, no difference between methotrexate and its silyl derivative could be seen. At a time when the cytotoxic lymphoblasts must have penetrated the blood brain barrier, only silyl methotrexate was able to prevent severe paralysis and death of the animals. It is suggested that the effect of N,N,O,O-Tetrakis (t-butyldimethylsilyl)-methotrexate, (N-4-N-(2,3-bis-t-butyldimethylsilyl-amino-6-pteridinyl-methyl)- methylamino-benzoyl-glutamic acid-bis-(t-butyldimethylsilyl)-ester), depends on the increased lipid solubility and permeability of the blood brain barrier of silylated drugs.

• Authors: H Przuntek, M E Westarp, M L Vohl, M Gerlach, P Jutzi, H Wekerle
• Journal: Neuropharmacology (Neuropharmacology) 1987 Feb-Mar Vol. 26 Issue 2-3 Pg. 255-60 ISSN: 0028-3908 [Print] England
• PMID: 3495744 (Publication Type: Comparative Study, Journal Article)

Chemical References

• N,N,O,O-tetrakis(tert-butyldimethylsilyl)methotrexate
• Methotrexate

Topics

• Animals
• Encephalomyelitis, Autoimmune, Experimental (drug therapy)
• Female
• Methotrexate (analogs & derivatives, therapeutic use)
• Mice
• Mice, Inbred Strains
• T-Lymphocytes (physiology)