Abstract |
Recombinant human interleukin-2 (rIL-2) suppressed metastatic tumour colony formation in the lungs of C57BL/6 mice bearing Lewis lung carcinoma (3LL). In tumour-bearing mice given rIL-2, non-specific killer cells that were cytotoxic not only against natural killer-sensitive YAC-1 cells but also against 3LL cells in an in vitro 51Cr-release assay were concomitantly induced as tumour metastasis was suppressed. These non-specific killer cells were mostly removed by treatment with anti-Thy 1.2 or anti- asialo GM1 antibody plus complement (C) in vitro but not with anti-Lyt 1.2 or anti-Lyt 2.2 plus C, indicating that they were positive for Thy 1 and asialo GM1 but not for Lyt 1 and Lyt 2. In order to explore the mechanism by which rIL-2 suppressed tumour metastasis, we examined the clearance of intravenously injected 51Cr-labelled 3LL cells in the lungs of mice given rIL-2. The rate of tumour cell clearance was increased. This enhanced clearance was almost completely removed by injecting anti- asialo GM1 antibody. In addition, the injection of anti- asialo GM1 antibody also depleted most of the non-specific killer cells induced by administering rIL-2. These results indicate that asialo GM1-positive cells are not only cytotoxic in vitro but also play a critical role in the clearance of 3LL cells in the lungs in vivo. Our results indicate that asialo GM1-positive cells play an important role as anti-metastatic effector cells in suppressing the metastasis of 3LL cells in mice given rIL-2.
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Authors | S Hinuma, K Naruo, K Ootsu, T Houkan, O Shiho, K Tsukamoto |
Journal | Immunology
(Immunology)
Vol. 60
Issue 2
Pg. 173-9
(Feb 1987)
ISSN: 0019-2805 [Print] England |
PMID | 3493209
(Publication Type: Journal Article)
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Chemical References |
- Antigens, Surface
- Glycosphingolipids
- Interleukin-2
- Recombinant Proteins
- G(M1) Ganglioside
- asialo GM1 ganglioside
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Topics |
- Animals
- Antigens, Surface
(analysis)
- Female
- G(M1) Ganglioside
- Glycosphingolipids
(immunology)
- Interleukin-2
(therapeutic use)
- Killer Cells, Natural
(immunology)
- Lung Neoplasms
(pathology, prevention & control, secondary)
- Mice
- Mice, Inbred C57BL
- Recombinant Proteins
(therapeutic use)
- Spleen
(immunology)
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