Induction of nonspecific cell-mediated cytotoxic reactivity from non-immune spleen cells treated with aloctin A.

Abstract

Culturing of mouse spleen cells with aloctin A (Alo A), a lectin having anti-tumor activity, resulted in the induction of cells cytotoxic to syngeneic and allogenic tumor cells in vitro. Alo A-induced killer cells could be generated from spleen cells of natural killer cell-deficient beige mice but not from those of T cell-deficient nude mice. The results of cytotoxicity assay after treatment with antisera plus complement indicated that the killer cells mainly consisted of Thy 1+, Lyt 1-2+, asialo GM1(1)-T cells. Their cytotoxic activity was markedly augmented by the presence of Alo A during the assay for cytotoxicity. The assay of IL2 in the culture fluid from Alo A-treated spleen cells and the effect of dexamethasone (DEX) on IL2 production from Alo A-stimulated spleen cells and on generation of killer cells suggested that generation of Alo A-induced killer cells was closely associated with the amount of IL2 released from the spleen cells in culture.

• Authors: K Imanishi, I Suzuki
• Journal: International journal of immunopharmacology (Int J Immunopharmacol) Vol. 8 Issue 7 Pg. 781-7 ( 1986) ISSN: 0192-0561 [Print] England
• PMID: 3491058 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Interleukin-1
• Lectins
• Mitogens
• Plant Lectins
• aloctin A
• Complement System Proteins

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Cell Line
• Complement System Proteins (immunology)
• Cytotoxicity, Immunologic (drug effects)
• Immunity, Cellular (drug effects)
• Interleukin-1 (biosynthesis)
• Lectins (pharmacology)
• Mice
• Mice, Inbred Strains
• Mitogens
• Neoplasms, Experimental (immunology)
• Plant Lectins
• Spleen (cytology, immunology)