Early rise of cytosolic Ca2+ induced by NGF in PC12 and chromaffin cells.

Abstract

A rise of cytosolic Ca2+ is induced by NGF in rat pheochromocytoma PC12 and bovine chromaffin cells investigated (both in suspension and while attached to polyornithine-coated glass slides) by fluorescence techniques (with quin-2 and fura-2). The effect of NGF on [Ca2+]i is delayed (30-40 s of lag phase), slow (t1/2 = 40 s), relatively small (+50-75%) and persistent (over 10 min). It is due to Ca2+ influx (requires extracellular Ca2+ greater than 10 microM) through a pathway different from the voltage-gated Ca2+ channel, possibly accompanied by intracellular Ca2+ redistribution, and might play a messenger role in NGF action.

• Authors: A Pandiella-Alonso, A Malgaroli, L M Vicentini, J Meldolesi
• Journal: FEBS letters (FEBS Lett) Vol. 208 Issue 1 Pg. 48-51 (Nov 10 1986) ISSN: 0014-5793 [Print] England
• PMID: 3490405 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aminoquinolines
• Benzofurans
• Fluorescent Dyes
• Nerve Growth Factors
• Epidermal Growth Factor
• Quin2
• Calcium
• Fura-2

Topics

• Adrenal Gland Neoplasms (metabolism)
• Aminoquinolines
• Animals
• Benzofurans
• Calcium (metabolism)
• Cattle
• Chromaffin System (metabolism)
• Cytosol (metabolism)
• Epidermal Growth Factor (pharmacology)
• Fluorescent Dyes
• Fura-2
• Nerve Growth Factors (pharmacology)
• Pheochromocytoma (metabolism)
• Rats