Abstract |
A rise of cytosolic Ca2+ is induced by NGF in rat pheochromocytoma PC12 and bovine chromaffin cells investigated (both in suspension and while attached to polyornithine-coated glass slides) by fluorescence techniques (with quin-2 and fura-2). The effect of NGF on [Ca2+]i is delayed (30-40 s of lag phase), slow (t1/2 = 40 s), relatively small (+50-75%) and persistent (over 10 min). It is due to Ca2+ influx (requires extracellular Ca2+ greater than 10 microM) through a pathway different from the voltage-gated Ca2+ channel, possibly accompanied by intracellular Ca2+ redistribution, and might play a messenger role in NGF action.
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Authors | A Pandiella-Alonso, A Malgaroli, L M Vicentini, J Meldolesi |
Journal | FEBS letters
(FEBS Lett)
Vol. 208
Issue 1
Pg. 48-51
(Nov 10 1986)
ISSN: 0014-5793 [Print] England |
PMID | 3490405
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Aminoquinolines
- Benzofurans
- Fluorescent Dyes
- Nerve Growth Factors
- Epidermal Growth Factor
- Quin2
- Calcium
- Fura-2
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Topics |
- Adrenal Gland Neoplasms
(metabolism)
- Aminoquinolines
- Animals
- Benzofurans
- Calcium
(metabolism)
- Cattle
- Chromaffin System
(metabolism)
- Cytosol
(metabolism)
- Epidermal Growth Factor
(pharmacology)
- Fluorescent Dyes
- Fura-2
- Nerve Growth Factors
(pharmacology)
- Pheochromocytoma
(metabolism)
- Rats
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