Radioimmunoassay of urinary 21-deoxytetrahydroaldosterone in primary aldosteronism and 21-hydroxylase deficiency.

Abstract

A radioimmunoassay of 21-deoxytetrahydroaldosterone was developed. Normal daily excretion of the unconjugated metabolite was 1.2 +/- 1.3 micrograms and of the glucuronized metabolite, 11.9 +/- 7 micrograms. The tetrahydroaldosterone/21-deoxytetrahydroaldosterone ratio varied more in patients with primary aldosteronism than in control subjects. Thus, measurements of the urinary excretion of the tetrahydroaldosterone or 21-deoxytetrahydroaldosterone alone did not provide an accurate expression for aldosterone production. Their sum correlated well with the clinical condition, i.e. clear-cut elevation in patients with primary aldosteronism. The diminished tetrahydroaldosterone/21-deoxytetrahydroaldosterone ratio found in patients with 21-hydroxylase deficiency may be attributed to increased bacterial conversion of tetrahydroaldosterone to 21-deoxytetrahydroaldosterone but could also stem from a deficiency implicating zona glomerulosa (aldosterone biosynthesis) regardless of the stage and clinical presentation of the disease.

• Authors: S Lewicka, J Winter, V D Bokkenheuser, P Vecsei, S Abdelhamid, U Heinrich
• Journal: Journal of steroid biochemistry (J Steroid Biochem) Vol. 24 Issue 2 Pg. 581-6 (Feb 1986) ISSN: 0022-4731 [Print] England
• PMID: 3486323 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Aldosterone
• 21-deoxytetrahydroaldosterone
• Steroid Hydroxylases

Topics

• Adrenal Hyperplasia, Congenital (urine)
• Aldosterone (analogs & derivatives, urine)
• Child
• Female
• Humans
• Hyperaldosteronism (urine)
• Male
• Radioimmunoassay
• Reference Values
• Steroid Hydroxylases (deficiency)