Abstract |
The present study was undertaken to examine cell cycle progression and SCE rate in three types of B-lymphoid cell line, viz., normal (KS-86), high-SCE Bloom syndrome (BS (BS2-2) and dimorphic BS (BS-SYW). In order to compare the dimorphic condition (BS-SYW) with artificial dimorphism (co-cultivation of BS2-2 with KS-86) these experiments were designed to test whether the BS B-lymphoid cell line cultures would influence the cell cycle progression and SCE rates of a normal B-lymphoid cell line, and vice versa. The present study resolved the controversy reported in the literature, by finding a definite time period under co-cultivation conditions when the SCE in normal cells was increased after 8 days of co-culture, whereas SCE in the BS cells decreased immediately with co-cultivation. In the dimorphic BS cell line (BS-SYW) the SCE frequency of a high-SCE cell population was also observed to be lower than that of a non-dimorphic BS cell line (BS2-2), thus corroborating the experimental observations under co-cultivation conditions. The decrease in BS SCE and increase in normal SCE (after a particular time period) is attributed to numerous causes discussed in relation to the cell cycle progression.
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Authors | R Bamezai, Y Shiraishi |
Journal | Experimental cell research
(Exp Cell Res)
Vol. 164
Issue 1
Pg. 163-73
(May 1986)
ISSN: 0014-4827 [Print] United States |
PMID | 3485532
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- B-Lymphocytes
(cytology)
- Bloom Syndrome
(genetics, pathology)
- Cell Cycle
- Cell Line
- Humans
- Kinetics
- Mitotic Index
- Sister Chromatid Exchange
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