Immunohistochemical study of the local inflammatory response to chlamydial ocular infection.

Abstract

Immunohistochemical staining of conjunctival biopsies from cynomolgus monkeys (Macaca fascicularis) was performed after they received a single primary ocular infection, a single secondary challenge infection, or repeated ocular inoculations with Chlamydia trachomatis. T cells of the suppressor/cytotoxic (OKT8F) phenotype predominated regardless of the infection protocol, and perifollicular T lymphocytes of both the suppressor/cytotoxic and helper (OKT4A) phenotypes appeared in large numbers during the peak inflammatory reaction. In repeatedly inoculated monkeys, T cells and follicles persisted until cessation of reinfection. IgM-bearing B lymphocytes comprised the majority of cells within follicles, with smaller numbers of IgG- or IgA-positive B cells. The major difference in the response to the various infection protocols was the increased number and persistence of follicles with repeated reinoculation. The finding of large numbers of T-suppressor/cytotoxic and T-helper cells in the infected conjunctiva supports a role for cell-mediated immunity in the local response to C. trachomatis ocular infection.

• Authors: J A Whittum-Hudson, H R Taylor, M Farazdaghi, R A Prendergast
• Journal: Investigative ophthalmology & visual science (Invest Ophthalmol Vis Sci) Vol. 27 Issue 1 Pg. 64-9 (Jan 1986) ISSN: 0146-0404 [Print] United States
• PMID: 3484473 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Immunoglobulins

Topics

• Animals
• B-Lymphocytes (classification, pathology)
• Biopsy
• Chlamydia Infections
• Chronic Disease
• Endophthalmitis (etiology, metabolism, pathology)
• Histocytochemistry
• Immunochemistry
• Immunoglobulins (classification, metabolism)
• Lymphoid Tissue (pathology)
• Macaca fascicularis
• Male
• T-Lymphocytes (classification, pathology)