Role of plasminogen activator inhibitor-1 in oral tongue squamous cell carcinoma: An immunohistochemical and in vitro analysis.

Abstract

OBJECTIVE:

To evaluate the influence of plasminogen activator inhibitor-1 (PAI-1) on the biological behavior and prognosis of oral tongue squamous cell carcinoma (OTSCC).

METHODS:

Immunoexpression of PAI-1 was analyzed in 60 OTSCC specimens and classified as low-expression (≤50% of positive cells) or high-expression (>50%). In vitro effects of recombinant human PAI-1 (rhPAI-1) were assessed through functional assays on the OTSCC-derived cell line SCC-25. Three cell groups were evaluated: G0 (control), G10 (10 nM rhPAI-1), and G20 (20 nM rhPAI-1).

RESULTS:

High membrane expression of PAI-1 was associated with tumor budding (p = 0.046) and high-risk cases (p = 0.043). Cytoplasmic and membrane expression of PAI-1 was not associated with patient survival. Cell viability (p = 0.020) and progression to the S-phase of the cell cycle (p = 0.024) were higher in G10 and G20 at 24 h. The percentages of apoptotic/necrotic cells were not affected by rhPAI-1. The presence of rhPAI-1 increased cell migration (p = 0.039) and invasion (p = 0.039) after 24 and 72 h, respectively.

CONCLUSION:

Our findings indicate the involvement of PAI-1 in the biological behavior of OTSCC, although its expression may not predict patient survival. The in vitro results suggest that PAI-1 stimulates cell proliferation, migration and invasion and may contribute to the aggressive phenotype of OTSCC.

• Authors: Rodrigo Porpino Mafra, Vladimir Galdino Sabino, Larissa Santos Amaral Rolim, Cyntia Helena Pereira de Carvalho, Cassiano Francisco Weege Nonaka, Carlos Augusto Galvão Barboza, Lélia Batista de Souza, Leão Pereira Pinto
• Journal: Experimental and molecular pathology (Exp Mol Pathol) Vol. 124 Pg. 104722 (02 2022) ISSN: 1096-0945 [Electronic] Netherlands
• PMID: 34800515 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier Inc. All rights reserved.

Chemical References

• Plasminogen Activator Inhibitor 1

Topics

• Adult
• Aged
• Aged, 80 and over
• Carcinoma, Squamous Cell (metabolism, pathology)
• Cell Line, Tumor
• Female
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• Plasminogen Activator Inhibitor 1 (metabolism)
• Prognosis
• Retrospective Studies
• Squamous Cell Carcinoma of Head and Neck (metabolism, pathology)
• Tongue Neoplasms (metabolism, pathology)