Pyruvate kinase M2 (PKM2), a glycolytic rate-limiting
enzyme, reportedly plays an important role in
tumorigenesis and the inflammatory response by regulating the metabolic reprogramming. However, its contribution to microglial activation during
neuroinflammation is still unknown. In this study, we observed an enhanced glycolysis level in the
lipopolysaccharide (LPS)-activated microglia. Utilizing the glycolysis inhibitor 2-DG, we proved that LPS requires glycolysis to induce microglial pyroptosis. Moreover, the
protein expression, dimer/monomer formation, phosphorylation and nuclear translocation of PKM2 were all increased by LPS. Silencing PKM2 or preventing its nuclear translocation by
TEPP-46 significantly alleviated the LPS-induced inflammatory response and pyroptosis in microglia. Employing
biological mass spectrometry combined with immunoprecipitation technology, we identified for the first time that PKM2 interacts with
activating transcription factor 2 (ATF2) in microglia. Inhibition of glycolysis or preventing PKM2 nuclear aggregation significantly reduced the phosphorylation and activation of ATF2. Furthermore, knocking down ATF2 reduced the LPS-induced pyroptosis of microglia. In vivo, we showed the LPS-induced pyroptosis in the cerebral cortex tissues of mice, and first found that an increased PKM2 expression was co-localized with ATF2 in the inflamed mice brain. Collectively, our data suggested for the first time that PKM2, a key rate-limiting
enzyme of the Warburg effect, directly interacts with the pro-inflammatory
transcription factor ATF2 to bridge glycolysis and pyroptosis in microglia, which might be a pivotal crosstalk between metabolic reprogramming and
neuroinflammation in the CNS.