Knockdown of lncRNA NEAT1 suppresses proliferation and migration, and induces apoptosis of cervical cancer cells by regulating the miR‑377/FGFR1 axis.

Abstract

To investigate the role of NEAT1 and the microRNA (miR)‑377/fibroblast growth factor receptor 1 (FGFR1) axis in cervical cancer (CC), the expression levels of NEAT1, FGFR1 and miR‑377 were detected in CC tissues and cell lines. NEAT1 or FGFR1 was knocked down by transfection with short hairpin RNA (sh)‑NEAT1 or sh‑FGFR1, and miR‑377 was overexpressed by transfection with miR‑377 mimics in HeLa and C33A cells. Cell viability and migration were measured using MTT and Transwell assays, respectively. Cell apoptosis was determined by flow cytometry. A dual luciferase reporter assay was performed to confirm the presence of binding sites between miR‑377 and FGFR1. The results revealed that the expression levels of NEAT1 and FGFR1 were significantly elevated, whereas miR‑377 expression was markedly decreased in CC tissues and cell lines. In HeLa and C33A cells, after NEAT1 knockdown, miR‑377 expression was increased, cell viability and migration were inhibited, and apoptosis was induced. Similarly, silencing FGFR1 inhibited cell viability and migration, and induced apoptosis of HeLa and C33A cells. A dual luciferase reporter gene assay verified a targeting relationship between NEAT1 and miR‑377. Inhibition of miR‑377 or overexpression of FGFR1 reversed the effects of NEAT1 knockdown on cell function in HeLa and C33A cells. Moreover, a dual luciferase reporter assay confirmed that FGFR1 was a direct target of miR‑377. In conclusion, suppression of NEAT1 inhibited cell viability and migration, and promoted apoptosis of CC cells, and these effects were achieved through regulation of the miR‑377/FGFR1 axis.

• Authors: Feng Geng, Wen-Cong Jia, Tao Li, Na Li, Wei Wei
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 25 Issue 1 (Jan 2022) ISSN: 1791-3004 [Electronic] Greece
• PMID: 34779493 (Publication Type: Journal Article)

Chemical References

• MIRN377 microRNA, human
• MicroRNAs
• NEAT1 long non-coding RNA, human
• RNA, Long Noncoding
• FGFR1 protein, human
• Receptor, Fibroblast Growth Factor, Type 1

Topics

• Aged
• Apoptosis (genetics)
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Cell Survival
• China
• Female
• Gene Expression (genetics)
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Primary Cell Culture
• RNA, Long Noncoding (genetics, metabolism)
• Receptor, Fibroblast Growth Factor, Type 1 (genetics, metabolism)
• Uterine Cervical Neoplasms (genetics, metabolism)