Differentiation-inducing factor for a human leukemic cell line produced by colony-stimulating factor producing human lung cancer tissue.

Abstract

We have found that medium conditioned by a colony-stimulating factor producing tumor derived from a granulocytosis case with lung cancer contained a factor to differentiate a human promyelocytic leukemic cell line (HL-60) to macrophage-like cells that were butyrate esterase-positive and had phagocytosing activity and membrane Fc receptors. This differentiation-inducing factor was not active for a human myeloblastic cell line (KG-1), and was separated from a colony-stimulating factor by its molecular weight and isoelectric point. The conditioned medium did not contain a detectable amount of gamma interferon when tested by bioassay as well as by radioimmunoassay. This is the first report that a human lung cancer tissue produces not only a colony-stimulating factor, but also a differentiation-inducing factor. The conditioned medium is considered to be a good source of differentiation-inducing factor.

• Authors: K Hatake, K Motoyoshi, T Hanamura, N Mukaida, T Kasahara, Y Miura, M Saito
• Journal: Experimental hematology (Exp Hematol) Vol. 15 Issue 9 Pg. 915-21 (Oct 1987) ISSN: 0301-472X [Print] Netherlands
• PMID: 3477467 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Culture Media
• Lymphokines
• monocyte-macrophage differentiation factor

Topics

• Animals
• Cell Differentiation (drug effects)
• Culture Media (analysis)
• Granulocytes (drug effects, pathology)
• Humans
• Leukemia, Myeloid, Acute (pathology)
• Leukocytosis (etiology)
• Lung Neoplasms (complications, metabolism, pathology)
• Lymphokines (isolation & purification, metabolism, pharmacology)
• Male
• Mice
• Mice, Inbred BALB C
• Neoplasm Transplantation
• Tumor Cells, Cultured (drug effects)
• Tumor Stem Cell Assay