Pairings and polarities of the 14 strands in sickle cell hemoglobin fibers.

Abstract

Sickle cell anemia results from the formation of hemoglobin S fibers in erythrocytes, and a greater understanding of the structure of these fibers should provide insights into the basis of the disease and aid in the development of effective antisickling agents. Improved reconstructions from electron micrographs of negatively stained single hemoglobin S fibers or embedded fiber bundles reveal that the 14 strands of the fiber are organized into pairs. The strands in each of the seven pairs are half-staggered, and from longitudinal views the polarity of each pair can be determined. The positions of the pairs and their polarities (three in one orientation; four in the opposite orientation) suggest a close relationship with the crystals of deoxyhemoglobin S composed of antiparallel pairs of half-staggered strands.

• Authors: D W Rodgers, R H Crepeau, S J Edelstein
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 84 Issue 17 Pg. 6157-61 (Sep 1987) ISSN: 0027-8424 [Print] United States
• PMID: 3476937 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Hemoglobin, Sickle

Topics

• Crystallization
• Hemoglobin, Sickle
• Humans
• Image Processing, Computer-Assisted
• Microscopy, Electron
• Protein Conformation