Equol is a metabolite of
daidzein, a major soybean
isoflavone with estrogenic and
antioxidant activities. As the production of
equol depends on the presence of certain members of the intestinal microflora, not all individuals can produce
equol. We examined the relationship between NASH histological features and
equol production. In an animal model, obese OLETF rats were intraperitoneally injected with a porcine serum to augment liver fibrogenesis.
Equol-rich soy product,
SE5-OH was orally administered during the experimental period. Treatment with
SE5-OH markedly attenuated the development of
liver fibrosis and expression of alpha-smooth muscle actin. In clinical research, 38
NAFLD patients (13 men and 25 women) were included. The degree of
fibrosis and ballooning in
equol-nonproducers was significantly higher than in
equol-producers in women. The percentage of nonproducers with
NAFLD activity score (
NAS) ≥ 5 was significantly higher than that of producers. None of the histological features were significantly different between nonproducers and producers in men. Decision tree analysis identified predictors for
NAS ≥ 5 in women. The status of
equol production was the strongest predictor, followed by fasting
glucose. Since
equol can be noninvasively detected in urine, it can be applied as a screening tool for the progression of NASH in women.