Differential endocytosis of fluorescein iso-thiocyanate-concanavalin A by normal and chronic myeloid leukemic granulocytes.

Abstract

Isolated granulocytes from normal individuals and patients suffering from chronic myeloid leukemia (CML) displayed different fluorescent patterns on treatment with fluorescein isothiocyanate concanavalin A (Fl-Con A). The ligand was internalized by 86% of the normal granulocytes, while 80% of the leukemic granulocytes exhibited Fl-Con A localized on the cell periphery. In further experiments, pretreatment of the normal granulocytes with cytochalasin B, iodoacetamide, 2-deoxyglucose and sodium fluoride (but not with sodium azide or dinitrophenol) was found to drastically inhibit internalization of the ligand. However, pretreatment of granulocytes from CML patients with cytochalasin B and 2-deoxyglucose, caused only a little alteration in the pattern of Fl-Con A labelling relative to untreated cells. These results indicate that CML granulocytes are defective in their ability to endocytose Fl-Con A. We suggest that this differential interaction between Fl-Con A and normal and leukemic granulocytes is a convenient system to study the initial steps in receptor mediated endocytosis of Concanavalin A.

• Authors: S M Zingde, P N Anklesaria, S H Advani, A N Bhisey, B P Gothoskar
• Journal: Blut (Blut) Vol. 55 Issue 2 Pg. 81-8 (Aug 1987) ISSN: 0006-5242 [Print] Germany
• PMID: 3475138 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Azides
• Fluoresceins
• fluorescein isothiocyanate-concanavalin A
• Concanavalin A
• Cytochalasin B
• Sodium Fluoride
• Sodium Azide
• Fluorescein-5-isothiocyanate
• Colchicine
• Iodoacetamide

Topics

• Azides (pharmacology)
• Cell Differentiation
• Cell Membrane (metabolism)
• Colchicine (pharmacology)
• Concanavalin A (analogs & derivatives, metabolism)
• Cytochalasin B (pharmacology)
• Endocytosis (drug effects)
• Fluorescein-5-isothiocyanate (analogs & derivatives)
• Fluoresceins (metabolism)
• Granulocytes (cytology, drug effects, metabolism)
• Histocytochemistry
• Humans
• Iodoacetamide (pharmacology)
• Kinetics
• Leukemia, Myeloid (blood, metabolism, physiopathology)
• Sodium Azide
• Sodium Fluoride (pharmacology)
• Time Factors