TG2-gluten complexes as antigens for gluten-specific and transglutaminase-2 specific B cells in celiac disease.

Abstract

A hallmark of celiac disease is the gluten-dependent production of antibodies specific for deamidated gluten peptides (DGP) and the enzyme transglutaminase 2 (TG2). Both types of antibodies are believed to result from B cells receiving help from gluten-specific CD4+ T cells and differentiating into antibody-producing plasma cells. We have here studied the collaboration between DGP- and TG2-specific B cells with gluten-specific CD4+ T cells using transgenic mice expressing celiac patient-derived T-cell and B-cell receptors, as well as between B-cell transfectants and patient-derived gluten-specific T-cell clones. We show that multivalent TG2-gluten complexes are efficient antigens for both TG2-specific and DGP-specific B cells and allow both types of B cells to receive help from gluten-specific T cells of many different specificities.

• Authors: Christian B Lindstad, Alisa E Dewan, Jorunn Stamnaes, Ludvig M Sollid, M Fleur du Pré
• Journal: PloS one (PLoS One) Vol. 16 Issue 11 Pg. e0259082 ( 2021) ISSN: 1932-6203 [Electronic] United States
• PMID: 34731200 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Antigen, B-Cell
• Glutens
• Gliadin
• Protein Glutamine gamma Glutamyltransferase 2

Topics

• Animals
• B-Lymphocytes (immunology, metabolism, pathology)
• CD4-Positive T-Lymphocytes (immunology, metabolism)
• Celiac Disease (genetics, immunology, pathology)
• Gliadin (genetics, immunology)
• Glutens (genetics, immunology)
• Humans
• Mice
• Mice, Transgenic
• Protein Glutamine gamma Glutamyltransferase 2 (genetics, immunology)
• Receptors, Antigen, B-Cell (genetics)
• T-Lymphocytes (immunology, metabolism, pathology)