Monoclonal antibodies to human osteosarcoma-associated antigen(s).

Abstract

Monoclonal antibodies (MoAbs) against human osteosarcoma cells were obtained by the production and cloning of hybrids resulting from the fusion of mouse myeloma cells P3 X 63Ag8.653 with spleen cells from partially purified, osteosarcoma-associated antigen (OSAA)-immunized BALB/c mice. OSAAs were isolated from the spent culture medium of a human osteosarcoma cell line (TE-85). Five hybrid clones were established and designated as OSA1, OSA2, OSA3, OSA4, and OSA5. OSA1 and OSA2 had similar activity. All 5 MoAbs reacted strongly with most osteosarcoma cell lines and with all osteosarcoma tissues tested but not with 10 tumor cell lines and 2 tumor tissues from other cancers. OSA3, OSA4, and OSA5 cross-reacted with a fibrosarcoma cell line, a colon cell line, and fibrosarcoma, respectively, as well as with a melanoma cell line. None of the MoAbs were reactive with activated normal human peripheral blood mononuclear cells (PBMC). Immunoprecipitation of membrane protein isolated from LM cells and TE-85 cells with the MoAbs OSA1 and OSA2 conjugated with Staphylococcus aureus yielded a molecule with molecular weight of approximately 92,000. No detectable membrane protein was precipitated when 125I-labeled membrane protein from pooled activated human PBMC and tumor cells of other histologic types were used in the immunoprecipitation.

• Authors: K Y Tsang, R Q Warren, L Bishop, S Pathak, B Koger, M F LaVia
• Journal: Journal of the National Cancer Institute (J Natl Cancer Inst) Vol. 77 Issue 6 Pg. 1175-80 (Dec 1986) ISSN: 0027-8874 [Print] United States
• PMID: 3467110 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigens, Neoplasm

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antibody Specificity
• Antigens, Neoplasm (analysis, immunology)
• Cell Line
• Humans
• Mice
• Mice, Inbred BALB C
• Osteosarcoma (immunology)