Abstract |
Lung cancer is a serious threat to human health due to its high morbidity and mortality. microRNAs ( miRNAs) are involved in the tumorigenesis and progression of lung cancer. In this study, we elucidated the role of miRNA-4507 (miR-4507) in the pathogenesis of non-small-cell lung cancer (NSCLC). miR-4507 is found to be upregulated in NSCLC cells (A549, H460). MTT, 5-ethynyl-2'-deoxyuridine (EdU), wound healing, and transwell assays were performed to evaluate NSCLC cell proliferation and migration. The results demonstrated that miR-4507 inhibition significantly decrease the proliferation and migration of NSCLC cells. Subsequently, a luciferase activity assay was conducted to verify the regulation of the predicted gene target of miR-4507, namely, TP53. Mechanism experiments show that miR-4507 activates the PI3K/AKT signal. Further, we co-transfected miR-4507 mimics and TP53 plasmids and found that TP53 overexpression could recover the effects of miR-4507 mimics on proliferation, migration, and the PI3K/AKT signal activation. These results suggested that miR-4507 targets TP53 to facilitate the proliferation and migration of lung cancer cells through PI3K/AKT signal and that miR-4507 could serve as a potential target for NSCLC treatment.
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Authors | MengYang Zhao, ZiBo Tang, YiJun Wang, JiaoJiao Ding, Ying Guo, Ning Zhang, TianHui Gao |
Journal | Journal of Cancer
(J Cancer)
Vol. 12
Issue 22
Pg. 6600-6609
( 2021)
ISSN: 1837-9664 [Print] Australia |
PMID | 34659550
(Publication Type: Journal Article)
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Copyright | © The author(s). |