Targeted Enrichment of Enzyme-Instructed Assemblies in Cancer Cell Lysosomes Turns Immunologically Cold Tumors Hot.

Abstract

Lysosome-relevant cell death induced by lysosomal membrane permeabilization (LMP) has recently attracted increasing attention. However, nearly no studies show that currently available LMP inducers can evoke immunogenic cell death (ICD) or convert immunologically cold tumors to hot. Herein, we report a LMP inducer named TPE-Py-pYK(TPP)pY, which can respond to alkaline phosphatase (ALP), leading to formation of nanoassembies along with fluorescence and singlet oxygen turn-on. TPE-Py-pYK(TPP)pY tends to accumulate in ALP-overexpressed cancer cell lysosomes as well as induce LMP and rupture of lysosomal membranes to massively evoke ICD. Such LMP-induced ICD effectively converts immunologically cold tumors to hot as evidenced by abundant CD8+ and CD4+ T cells infiltration into the cold tumors. Exposure of ALP-catalyzed nanoassemblies in cancer cell lysosomes to light further intensifies the processes of LMP, ICD and cold-to-hot tumor conversion. This work thus builds a new bridge between lysosome-relevant cell death and cancer immunotherapy.

• Authors: Shenglu Ji, Jun Li, Xingchen Duan, Jingtian Zhang, Yufan Zhang, Mengqing Song, Songge Li, Hongli Chen, Dan Ding
• Journal: Angewandte Chemie (International ed. in English) (Angew Chem Int Ed Engl) Vol. 60 Issue 52 Pg. 26994-27004 (12 20 2021) ISSN: 1521-3773 [Electronic] Germany
• PMID: 34643312 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021 Wiley-VCH GmbH.

Chemical References

• Antineoplastic Agents
• Organophosphates
• Hydroxyl Radical
• Alkaline Phosphatase

Topics

• Alkaline Phosphatase (metabolism)
• Animals
• Antineoplastic Agents (chemical synthesis, metabolism, radiation effects, therapeutic use)
• Cell Line, Tumor
• Drug Design
• HEK293 Cells
• Humans
• Hydroxyl Radical (metabolism)
• Immunogenic Cell Death (drug effects)
• Intracellular Membranes (metabolism)
• Light
• Lysosomes (enzymology, metabolism)
• Mice
• Neoplasms (drug therapy)
• Organophosphates (chemical synthesis, metabolism, radiation effects, therapeutic use)
• Permeability (drug effects)