NLRP3
inflammasome-mediated pyroptosis is a proinflammatory programmed cell death pathway, which plays a vital role in functional outcomes after
stroke. We previously described the beneficial effects of
curcumin against
stroke-induced neuronal damage through modulating microglial polarization. However, the impact of
curcumin on microglial pyroptosis remains unknown. Here,
stroke was modeled in mice by
middle cerebral artery occlusion (MCAO) for 60 minutes and treated with
curcumin (150 mg/kg) intraperitoneally immediately after reperfusion, followed by daily administrations for 7 days.
Curcumin ameliorated white matter (WM) lesions and brain tissue loss 21 days poststroke and improved sensorimotor function 3, 10, and 21 days after
stroke. Furthermore,
curcumin significantly reduced the number of gasdermin D+ (GSDMD+) Iba1+ and
caspase-1+Iba1+ microglia/macrophage 21 days after
stroke. In vitro,
lipopolysaccharide (LPS) with
ATP treatment was used to induce pyroptosis in primary microglia. Western blot revealed a decrease in pyroptosis-related
proteins, e.g., GSDMD-N, cleaved caspase-1, NLRP3, IL-1β, and
IL-18, following in vitro or in vivo
curcumin treatment. Mechanistically, both in vivo and in vitro studies confirmed that
curcumin inhibited the activation of the NF-κB pathway. NLRP3 knocked down by
siRNA transfection markedly increased the inhibitory effects of
curcumin on microglial pyroptosis and proinflammatory responses, both in vitro and in vivo. Furthermore, stereotaxic microinjection of AAV-based NLRP3
shRNA significantly improved sensorimotor function and reduced WM lesion following
curcumin treatment in MCAO mice. Our study suggested that
curcumin reduced
stroke-induced WM damage, improved functional outcomes, and attenuated microglial pyroptosis, at least partially, through suppression of the NF-κB/NLRP3 signaling pathway, further supporting
curcumin as a potential therapeutic
drug for
stroke.