Pluripoietin alpha: a second human hematopoietic colony-stimulating factor produced by the human bladder carcinoma cell line 5637.

Abstract

We have identified a factor constitutively produced by the human bladder carcinoma cell line 5637, which has the following capacities: to induce the differentiation of the human promyelocytic leukemic cell line HL-60; to induce the expression of chemotactic peptide receptors in leukemic cells as well as in normal peripheral blood granulocytes; to function as a chemoattractant for neutrophils as well as, under certain conditions, inhibiting their migration; to support the growth of eosinophil and granulocyte/macrophage progenitors, which is maximal when scored at day 14; and to support the growth of human mixed pluripotent progenitors and erythroid bursts from normal human bone marrow. This factor has a molecular size of 32 kDa by gel filtration and 16.5 kDa by NaDodSO4/PAGE. We have termed this factor pluripoietin alpha.

• Authors: J L Gabrilove, K Welte, P Harris, E Platzer, L Lu, E Levi, R Mertelsmann, M A Moore
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 83 Issue 8 Pg. 2478-82 (Apr 1986) ISSN: 0027-8424 [Print] United States
• PMID: 3458209 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Growth Substances
• Neoplasm Proteins
• Receptors, Formyl Peptide
• Receptors, Immunologic

Topics

• Bone Marrow Cells
• Carcinoma (analysis)
• Cell Differentiation
• Cell Line
• Chemotaxis, Leukocyte
• Erythropoiesis
• Growth Substances (isolation & purification, physiology)
• Hematopoiesis
• Hematopoietic Stem Cells (cytology)
• Humans
• Molecular Weight
• Neoplasm Proteins (isolation & purification, physiology)
• Neoplastic Stem Cells (cytology, physiology)
• Receptors, Formyl Peptide
• Receptors, Immunologic (metabolism)
• Urinary Bladder Neoplasms (analysis)