Expression of murine gamma fetal antigen in adult hematopoietic tissue and during induced differentiation of Friend erythroleukemia cells.

Abstract

Quantitative analysis of extracts of various normal adult CD-1 mouse tissues indicated that the serologically defined murine gamma fetal antigen (gamma-FA) was expressed at high levels in hematopoietic tissue in general and in bone marrow (BM) in particular. Metabolic labeling of isolated BM cells indicated that the BM was a site of gamma-FA synthesis in the adult animal. The size(s) of the antigen immunoprecipitated from labeled BM cells (35 and 27 kilodaltons) with anti-gamma-FA serum correlated well with molecular weight estimates of fibrosarcoma-fetal mouse-associated gamma-FA, as determined by molecular sieve chromatography. For ascertainment of the relationship between hematopoietic cell differentiation and gamma-FA content, a multiparameter flow cytometric approach was used to evaluate gamma-FA levels in Friend erythroleukemia (FL) cells as a function of growth state (blast or dimethyl sulfoxide-differentiated) and cell-cycle compartment. Differentiated G1-arrested FL cells (G1D) possessed significantly lower gamma-FA-associated immunofluorescence as compared to control cells in the G0-G1 substate. Remaining S- and G2 + M-phase cells in differentiated populations demonstrated an even greater reduction in gamma-FA content relative to control cells in the corresponding cell-cycle phases. The available data support the tentative classification of gamma-FA as a murine differentiation antigen.

• Authors: P J Higgins, A E Silverstone, C Bueti, V F Pizzi, M R Melamed, M Lipkin, F Traganos
• Journal: Journal of the National Cancer Institute (J Natl Cancer Inst) Vol. 76 Issue 5 Pg. 885-93 (May 1986) ISSN: 0027-8874 [Print] United States
• PMID: 3457976 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Actins
• Antigens, Neoplasm
• Fetal Proteins
• Sulfur Radioisotopes
• gamma-fetal antigen
• DNA
• Methionine
• Dimethyl Sulfoxide

Topics

• Actins (analysis)
• Animals
• Antigens, Neoplasm (analysis, immunology)
• Cell Cycle
• Cell Differentiation (drug effects)
• Cell Line
• Chemical Precipitation
• DNA (analysis)
• Dimethyl Sulfoxide (pharmacology)
• Fetal Proteins (analysis, immunology)
• Friend murine leukemia virus
• Hematopoietic System (immunology)
• Leukemia, Erythroblastic, Acute (immunology, pathology)
• Leukemia, Experimental (immunology)
• Methionine (metabolism)
• Mice
• Sulfur Radioisotopes