Abstract | BACKGROUND: In adult rats we study the short- and long-term effects of focal blue light-emitting diode (LED)-induced phototoxicity (LIP) on retinal thickness and Iba-1+ activation. METHODS: The left eyes of previously dark-adapted Sprague Dawley (SD) rats were photoexposed to a blue LED (20 s, 200 lux). In vivo longitudinal monitoring of retinal thickness, fundus images, and optical retinal sections was performed from 1 to 30 days (d) after LIP with SD-OCT. Ex vivo, we analysed the population of S-cone and Iba-1+ cells within a predetermined fixed-size circular area (PCA) centred on the lesion. RESULTS: LIP resulted in a circular focal lesion readily identifiable in vivo by fundus examination, which showed within the PCAs a progressive thinning of the outer retinal layer, and a diminution of the S-cone population to 19% by 30 d. In parallel to S-cone loss, activated Iba-1+ cells delineated the lesioned area and acquired an ameboid morphology with peak expression at 3 d after LIP. Iba-1+ cells adopted a more relaxed-branched morphology at 7 d and by 14-30 d their morphology was fully branched. CONCLUSION: LIP caused a progressive reduction of the outer retina with loss of S cones and a parallel dynamic activation of microglial cells in the lesioned area.
|
Authors | Juan A Miralles de Imperial-Ollero, Alejandro Gallego-Ortega, María Norte-Muñoz, Johnny Di Pierdomenico, José Manuel Bernal-Garro, Francisco J Valiente-Soriano, Manuel Vidal-Sanz |
Journal | International journal of molecular sciences
(Int J Mol Sci)
Vol. 22
Issue 18
(Sep 09 2021)
ISSN: 1422-0067 [Electronic] Switzerland |
PMID | 34575905
(Publication Type: Journal Article)
|
Chemical References |
|
Topics |
- Animals
- Biomarkers
- Disease Models, Animal
- Disease Susceptibility
- Fluorescent Antibody Technique
- Light
- Microglia
(metabolism, pathology, radiation effects)
- Rats
- Retina
(metabolism, pathology, radiation effects)
- Retinal Cone Photoreceptor Cells
(metabolism, pathology, radiation effects)
- Retinal Degeneration
(diagnostic imaging, etiology, metabolism, pathology)
- Time Factors
- Tomography, Optical Coherence
|