Characteristics of macrophages from myelodysplastic syndrome microenvironment.

Abstract

Myelodysplastic syndrome (MDS) is a heterogeneous group of clonal hematopoietic neoplasms. The progression of malignancy is closely associated with immune regulation. Macrophages are indispensable tissue components and have been proposed to play a role in the pathophysiology of hematopoietic malignancies. However, the specific role of macrophages in the development of MDS remains unclear. Here, we investigated the characteristics and phenotypic evolution of macrophages from patients with MDS. Macrophages from patients with MDS expressed CD68, CD86 and CD163. Furthermore, MDS macrophages exhibited more M2-related characteristics. Moreover, a number of phenotype-associated genes in MDS macrophages exhibited diverse responses to iron overload or iron chelation upon stimulation by ferric chloride or deferoxamine (DFO, an iron chelator). Ferric chloride polarized MDS macrophages to exhibit more M1-related characteristics, a phenomenon that could be partially reversed by DFO. Therefore, this study reveals the characteristics and phenotypic evolution of MDS macrophages and broadens the knowledge of macrophage plasticity in hematopoietic malignancies.

• Authors: Feifei Yang, Zhaoxian Wu, Dan Yang, Xiuqun Zhang, Xuezhong Zhang, Yanli Xu
• Journal: Experimental cell research (Exp Cell Res) Vol. 408 Issue 1 Pg. 112837 (11 01 2021) ISSN: 1090-2422 [Electronic] United States
• PMID: 34547255 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier Inc. All rights reserved.

Chemical References

• Antigens, CD
• Antigens, Differentiation, Myelomonocytic
• CD163 antigen
• Chlorides
• Ferric Compounds
• Receptors, Cell Surface
• Iron
• ferric chloride

Topics

• Adult
• Aged
• Antigens, CD (metabolism)
• Antigens, Differentiation, Myelomonocytic (metabolism)
• Cellular Microenvironment (physiology)
• Chlorides (metabolism)
• Female
• Ferric Compounds (metabolism)
• Humans
• Iron (metabolism)
• Iron Overload (metabolism, pathology)
• Macrophages (pathology)
• Male
• Middle Aged
• Myelodysplastic Syndromes (pathology)
• Receptors, Cell Surface (metabolism)