Using HE, AB/PAS,
HID/AB, PAT/KOH/PAS
stain and CEA
monoclonal antibody BA
stain techniques, composition of
mucin and distribution of CEA in the
tumor tissue were detected in 65 cases of colorectal tubular
adenoma, 7
adenoma with malignant change and 14 established
tubular adenocarcinoma in order to study the functional changes of colorectal
adenoma and its relation with the colorectal
adenocarcinoma. According to Konishi's criteria, colorectal tubular
adenomas were divided into three groups: mild, moderate and severe dysplasia, added with two of ours:
adenoma with malignant change and established
adenocarcinoma, totalling 5 groups for comparison. The results showed that CEA positive rate increased with the grade of dysplasia in these 5 groups (33%, 57%, 67%, 86% and 100%). Similarly, the percentage of non-acetylated
acid and C7 C9 O-acetylated
sialomucin also gradually increased with the grade of dysplasia, showing a parallel tendency. Epithelial cells, secreting a heterogeneous
mucin, could be taken as a signal of abnormal cellular differentiation. The increase of non-acetylated
acid and C7 C9 O-acetylated
sialomucin in colorectal
adenoma implies a likelihood of malignant change.