Circular RNA Circ_0000677 promotes cell proliferation by regulating microRNA-106b-5p/CCND1 in non-small cell lung cancer.

Abstract

Recently, circular RNAs (circRNAs) have become an intense focus of research and large numbers of circRNAs have been identified, awaiting functional elucidation. Thus, the present study aims to examine the regulation of circRNAs and its molecular mechanism in lung cancer growth. Here, we show that circular RNA circ_0000677 was overexpressed and correlated with poor prognosis in non-small cell lung cancer (NSCLC) patients. Functionally, circ_0000677 knockdown markedly inhibited proliferation of NSCLC cells by observing of immunofluorescence staining of Ki67, clone formation assay, and xenograft experiments. In mechanism, circ_0000677 acted as a sponge of microRNA-106b and further regulated CCDND1 gene expression in NSCLC cells by dual luciferase activity assay and their expression examination. Taken together, these findings suggest a role for circ_0000677/miR-106b/CCND1 regulation axis in promoting NSCLC growth and progression.

• Authors: Xin Hu, Ping Wang, Chen Qu, Haibo Zhang, Liang Li
• Journal: Bioengineered (Bioengineered) Vol. 12 Issue 1 Pg. 6229-6239 (12 2021) ISSN: 2165-5987 [Electronic] United States
• PMID: 34519258 (Publication Type: Journal Article)

Chemical References

• CCND1 protein, human
• MIRN106 microRNA, human
• MicroRNAs
• RNA, Circular
• Cyclin D1

Topics

• Animals
• Carcinoma, Non-Small-Cell Lung (genetics, metabolism, mortality)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Cyclin D1 (genetics, metabolism)
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• Lung (metabolism)
• Lung Neoplasms (genetics, metabolism, mortality)
• Male
• Mice
• Mice, Inbred BALB C
• MicroRNAs (genetics, metabolism)
• RNA, Circular (genetics, metabolism)