Computational investigation of the binding of a designed peptide to λ light chain amyloid fibril.

Abstract

Systemic light chain amyloidosis (AL) causes a malignant pathology associated with the formation of amyloid fibrils that deposit in human organs and tissues, leading to dysfunction and severe morbidity. Amyloid fibril-reactive antibodies have been used to remove amyloid from organs and are effective in restoring organ function in patients with AL amyloidosis. Unfortunately, antibodies do not bind amyloid in all AL patients, nor do they efficiently bind many other forms of amyloid. Recently, a synthetic peptide P62 was developed, which binds many forms of systemic amyloidosis and can be further modified and fused to a high-affinity peptide epitope to expand its utility as a novel amyloid immunotherapeutic. However, the molecular-level details of P62-fibril binding mechanisms, critical for future peptide design, are unclear. Here, we combine protein docking, all-atom molecular dynamics simulation and umbrella sampling to study the dynamical interactions between peptide P62 and a structural model of the λ light chain in systemic amyloidosis. We found that P62 only binds to the canonical interface of the fibril where the peptide inserts into the fibril groove and its two termini are more mobile than the helix core. Our results also revealed an important role of the lysine residues of P62 in the binding process by forming initial contacts with aspartic acids on the fibril surface. Collectively, our computational study provided molecular-level insights into the binding mechanism between an amyloid fibril model and peptide P62, which could lay a foundation for rational design of peptides for improved amyloid diagnosis and immunotherapy.

• Authors: Zhenyu Wang, Wanying Huang, Manjun Liu, Stephen J Kennel, Jonathan S Wall, Xiaolin Cheng
• Journal: Physical chemistry chemical physics : PCCP (Phys Chem Chem Phys) Vol. 23 Issue 36 Pg. 20634-20644 (Sep 22 2021) ISSN: 1463-9084 [Electronic] England
• PMID: 34515265 (Publication Type: Journal Article)

Chemical References

• Amyloidogenic Proteins
• Peptides

Topics

• Amyloidogenic Proteins (chemistry)
• Humans
• Molecular Dynamics Simulation
• Peptides (chemical synthesis, chemistry)
• Protein Binding
• Protein Conformation