Abstract |
The objective of this study was to determine the expression and functional role of a long noncoding RNA ( lncRNA) MIAT ( myocardial infarction-associated transcript) in leiomyoma pathogenesis. Leiomyoma compared with myometrium (n = 66) expressed significantly more MIAT that was independent of race/ethnicity and menstrual cycle phase but dependent on MED12 ( mediator complex subunit 12) mutation status. Leiomyomas bearing the MED12 mutation expressed higher levels of MIAT and lower levels of microRNA 29 family (miR-29a, -b, and -c) compared with MED12 wild-type leiomyomas. Using luciferase reporter activity and RNA immunoprecipitation analysis, MIAT was shown to sponge the miR-29 family. In a 3-dimensional spheroid culture system, transient transfection of MIAT siRNA in leiomyoma smooth muscle cell (LSMC) spheroids resulted in upregulation of miR-29 family and downregulation of miR-29 targets, collagen type I (COL1A1), collagen type III (COL3A1), and TGF-β3 ( transforming growth factor β-3). Treatment of LSMC spheroids with TGF-β3 induced COL1A1, COL3A1, and MIAT levels, but repressed miR-29 family expression. Knockdown of MIAT in LSMC spheroids blocked the effects of TGF-β3 on the induction of COL1A1 and COL3A1 expression. Collectively, these results underscore the physiological significance of MIAT in extracellular matrix accumulation in leiomyoma.
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Authors | Tsai-Der Chuang, Derek Quintanilla, Drake Boos, Omid Khorram |
Journal | Endocrinology
(Endocrinology)
Vol. 162
Issue 11
(11 01 2021)
ISSN: 1945-7170 [Electronic] United States |
PMID | 34491311
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | © The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: [email protected]. |
Chemical References |
- MIRN29a microRNA, human
- Miat long non-coding RNA
- MicroRNAs
- RNA, Long Noncoding
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Topics |
- Adult
- Cells, Cultured
- Down-Regulation
(genetics)
- Extracellular Matrix
(metabolism)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Leiomyoma
(genetics, metabolism, pathology)
- MicroRNAs
(genetics)
- Middle Aged
- Multigene Family
(genetics)
- Protein Multimerization
(genetics)
- RNA, Long Noncoding
(physiology)
- Uterine Neoplasms
(genetics, metabolism, pathology)
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