CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene.

Abstract

SUV39H1 is a histone methyltransferase involve numerous biological processes, including of aging, embryo development, tumor growth and mitosis via catalysis of dimethylation and trimethylation of lysine 9 of histone H3. Here we report a human induced pluripotent stem cell line (EHTJUi005-A-1) which is generated from a wildtype human iPSC previously established in our laboratory, and this iPSC has a homozygous knockout of 8 bp in Exon 2 of SUV39H1. This iPSC model provides a valuable resource to study epigenetic regulation in extensive biological processes as mentioned above.

• Authors: Zhi-Bin Qiao, Ji-Zhen Lu, Lu Zhang, Hong-Xia Cao, Zhi-Hui Bai, Yi-Yao Qi, Han-Yu Zhu, Shou-Mei Zhang, Yan Bao, Wen-Wen Jia, Zhong-Min Liu
• Journal: Stem cell research (Stem Cell Res) Vol. 56 Pg. 102519 (10 2021) ISSN: 1876-7753 [Electronic] England
• PMID: 34464854 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021. Published by Elsevier B.V.

Chemical References

• Histones
• Repressor Proteins
• SUV39H1 protein, human
• Histone Methyltransferases
• Methyltransferases

Topics

• CRISPR-Cas Systems (genetics)
• Epigenesis, Genetic
• Histone Methyltransferases
• Histones (genetics, metabolism)
• Humans
• Induced Pluripotent Stem Cells (metabolism)
• Methyltransferases (genetics, metabolism)
• Repressor Proteins (genetics)