[Mechanism of the qualitative modification of mitochondrial monoamine oxidase activity by pyrazidol].

Abstract

Inhibitory effect of pyrazidol (I) on serotonin-deaminating activity in mice brain mitochondria, which was accompanied by induction in this source of monoamine oxidase (MAO) of a property to oxidize histamine, was distinct from the effect of 3,3a-dehydroderivative of pyrazidol (II), which did not induced the histamine deaminating activity. Analysis of electron absorption spectra and polarographic studies demonstrated that inhibition of MAO by pyrazidol resulted in dehydration of the piperazine ring in the molecule of the drug as well as in possible formation of azomethine bond at the 2-3 position, exhibiting high oxidizing activity. At the same time, marked alterations in the substance II structure were not found in the course of inhibition of serotonin-deaminating activity in mitochondria. As pyrazidol did not exhibit pro-oxidant activity, its azomethine group appears to have a direct oxidating activity, which was not mediated via lipid peroxides.

• Authors: E A Baumanis, I A Birska, G O Reĭkman, I E Kirule, V I Shvedov
• Journal: Voprosy meditsinskoi khimii (Vopr Med Khim) 1987 Nov-Dec Vol. 33 Issue 6 Pg. 90-6 ISSN: 0042-8809 [Print] Russia (Federation)
• Vernacular Title: O mekhanizme kachestvennoĭ modifikatsii pirazidolom aktivnosti mitokhondrial'noĭ monoaminoksidazy.
• PMID: 3445553 (Publication Type: English Abstract, Journal Article)

Chemical References

• Carbazoles
• Monoamine Oxidase Inhibitors
• Serotonin
• Histamine
• pirlindole

Topics

• Animals
• Brain (enzymology, metabolism)
• Carbazoles (metabolism, pharmacology)
• Deamination
• Histamine (metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• Mitochondria (enzymology, metabolism)
• Monoamine Oxidase Inhibitors (metabolism, pharmacology)
• Oxidation-Reduction
• Polarography
• Serotonin (metabolism)
• Spectrum Analysis