Abstract | BACKGROUND: METHODS:
Ribonuclease R ( RNase R) and subcellular localization assays were utilized to characterize circ_0099999. The levels of circ_0099999, microRNA (miR)-330-5p, and fascin actin-bundling protein 1 (FSCN1) were gauged by quantitative real-time PCR (qRT-PCR) and western blot. Cell proliferation, colony formation, apoptosis, migration, and invasion were evaluated by the Cell Counting Kit-8 (CCK-8), colony formation, flow cytometry, and transwell assays, respectively. The levels of glucose consumption and lactate production were determined using the assay kits. A direct relationship between miR-330-5p and circ_0099999 or FSCN1 was validated by dual- luciferase reporter assay. Tumour xenograft assays were used to analyse the role of circ_0099999 in vivo. RESULTS: Circ_0099999 was highly up-regulated in pancreatic cancer tissues and cells. Knockdown of circ_0099999 impeded cell proliferation, migration, invasion, glycolysis, and promoted apoptosis in vitro, as well as diminished tumour growth in vivo. Circ_0099999 targeted miR-330-5p, and miR-330-5p was a downstream mediator of circ_0099999 function. FSCN1 was a direct and functional target of miR-330-5p. Furthermore, circ_0099999 operated as a ceRNA for miR-330-5p to modulate FSCN1 expression. CONCLUSIONS: Our findings established a novel causal mechanism, circ_0099999/miR-330-5p/FSCN1 ceRNA crosstalk, in regulating pancreatic carcinogenesis and provided that inhibition of circ_0099999 might have therapeutic benefits in pancreatic cancer.
|
Authors | Yang Wang, Feng Zhang, Dongde Wu, Qun Wang, Lei Nie, Jing Yu |
Journal | Autoimmunity
(Autoimmunity)
Vol. 54
Issue 7
Pg. 471-482
(Nov 2021)
ISSN: 1607-842X [Electronic] England |
PMID | 34409897
(Publication Type: Journal Article)
|
Chemical References |
- Carrier Proteins
- FSCN1 protein, human
- MIRN330 microRNA, human
- MicroRNAs
- Microfilament Proteins
- RNA, Circular
|
Topics |
- Apoptosis
(genetics)
- Carrier Proteins
(genetics)
- Cell Proliferation
(genetics)
- Female
- Humans
- Male
- MicroRNAs
(genetics)
- Microfilament Proteins
(genetics)
- Pancreatic Neoplasms
(genetics)
- RNA, Circular
(genetics)
|