Interaction of the antitumor Au(I) complex [Au(Ph2P(CH2)2PPh2)2]Cl with human blood plasma, red cells, and lipoproteins: 31P and 1H NMR studies.

Abstract

The bis-chelated tetrahedral gold(I) complex [Au(dppe)2]Cl, where dppe is Ph2P(CH2)2PPh2, is active in several animal tumor models. When added to human blood plasma in vitro it appears to bind to lipoproteins, giving a slightly broadened 31P NMR signal, and 1H NMR resonances which are too broad to detect. Some lipoprotein is denatured. 31P NMR studies suggest that some [Au(dppe)2]+ is transferred from plasma to red cells with a half-life of ca. 2 hr. The complex binds within red cell membranes and the 1H resonances of intracellular glutathione are unaffected. The 31P NMR resonance from [Au(dppe)2]+ in red cell membranes is observable only when the complex is mobilized by addition of sodium dodecyl sulphate, which also mobilizes membrane phospholipids.

• Authors: S J Berners-Price, P J Sadler
• Journal: Journal of inorganic biochemistry (J Inorg Biochem) Vol. 31 Issue 4 Pg. 267-81 (Dec 1987) ISSN: 0162-0134 [Print] United States
• PMID: 3437279 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Lipoproteins
• Organogold Compounds
• Organometallic Compounds
• Organophosphorus Compounds
• Phosphorus
• bis(1,2-bis(diphenylphosphino)ethane)gold(I)
• Hydrogen

Topics

• Antineoplastic Agents (blood)
• Chemical Precipitation
• Erythrocytes (metabolism)
• Humans
• Hydrogen
• Kinetics
• Lipoproteins (blood)
• Magnetic Resonance Spectroscopy (methods)
• Organogold Compounds
• Organometallic Compounds (blood)
• Organophosphorus Compounds (blood)
• Phosphorus
• Protein Binding