Abstract |
In this study, an inulin fructan (TMP50-2) with moderate anti- tumor activity was obtained from dandelion. To further improve the anti- tumor activity of TMP50-2, a monodisperse and stable spherical nanoparticle (Tw-TMP-SeNP, 50 nm) was fabricated. Physico-chemical analysis revealed that TMP50-2 and Tween 80 were tightly wrapped on the surface of SeNPs by forming CO⋯Se bonds or through hydrogen bonding interaction (OH⋯Se). In vitro anti- tumor assay showed that Tw- TMP- SeNP treatment could significantly inhibit the proliferation of cancer cells (HepG2, A549, and HeLa) in a dose-dependent manner, while HepG2 cells were more susceptible to Tw- TMP- SeNP with an IC50 value of 46.8 μg/mL. The apoptosis induction of HepG2 cells by Tw- TMP- SeNP was evidenced by increasing the proportion of apoptotic cells ranging from 12.5% to 27.4%. Furthermore, in vivo zebrafish model confirmed the anti- tumor activity of Tw- TMP- SeNP by inhibiting the proliferation and migration of tumor cells as well as the angiogenesis of zebrafish embryos.
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Authors | Shaojie Zhang, Ziteng Song, Lijuan Shi, Linan Zhou, Jie Zhang, Jianlin Cui, Yuhao Li, Da-Qing Jin, Yasushi Ohizumi, Jing Xu, Yuanqiang Guo |
Journal | Carbohydrate polymers
(Carbohydr Polym)
Vol. 270
Pg. 118365
(Oct 15 2021)
ISSN: 1879-1344 [Electronic] England |
PMID | 34364610
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Elsevier Ltd. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- Fructans
- Polysaccharides
- Selenium
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Topics |
- A549 Cells
- Animals
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Cell Proliferation
(drug effects)
- Fructans
(chemistry, pharmacology)
- HeLa Cells
- Hep G2 Cells
- Humans
- Hydrogen Bonding
- Nanoparticles
(chemistry)
- Neoplasms
(drug therapy, metabolism, pathology)
- Polysaccharides
(chemistry, pharmacology)
- Selenium
(chemistry, pharmacology)
- Taraxacum
(chemistry)
- Zebrafish
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