Glucose-Targeted Hydroxyapatite/Indocyanine Green Hybrid Nanoparticles for Collaborative Tumor Therapy.

Abstract

Nanoscale hydroxyapatite (nHA) is considered as a promising drug carrier or therapeutic agent against malignant tumors. But the strong agglomeration tendency and lack of active groups seriously hamper their usage in vivo. To address these issues, we fabricated an organic-inorganic hybrid nanosystem composed of poly(acrylic acid) (PAA), nHA, and indocyanine green (ICG), and further modified with glucose to give a targeting nanosystem (GA@HAP/ICG-NPs). These hybrid nanoparticles (∼90 nm) showed excellent storage and physiological stability assisted by PAA and had a sustained drug release in an acidic tumor environment. In vitro cell experiments confirmed that glucose-attached particles significantly promoted cellular uptake and increased intracellular ICG and Ca2+ concentrations by glucose transporter 1 (GLUT1)-mediated endocytosis. Subsequently, the excessive Ca2+ induced cell or organelle damage and ICG triggered photothermal and photodynamic effects (PTT/PDT) under laser irradiation, resulting in enhanced cell toxicity and apoptosis. In vivo tests revealed that the hybrid nanosystem possessed good hemocompatibility and biosafety, facilitating in vivo circulation and usage. NIR imaging further showed that tumor tissues had more drug accumulation, resulting in the highest tumor growth inhibition (87.89%). Overall, the glucose-targeted hybrid nanosystem was an effective platform for collaborative therapy and expected to be further used in clinical trials.

• Authors: Xu Cheng, Yingran Xu, Yong Zhang, Chaochao Jia, Bing Wei, Ting Hu, Rupei Tang, Conghu Li
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 13 Issue 31 Pg. 37665-37679 (Aug 11 2021) ISSN: 1944-8252 [Electronic] United States
• PMID: 34342216 (Publication Type: Journal Article)

Chemical References

• Acrylic Resins
• Antineoplastic Agents
• Drug Carriers
• Photosensitizing Agents
• carbopol 940
• Durapatite
• Indocyanine Green
• Glucose
• Calcium

Topics

• Acrylic Resins (chemistry, toxicity)
• Animals
• Antineoplastic Agents (chemistry, therapeutic use, toxicity)
• Apoptosis (drug effects)
• Calcium (metabolism)
• Drug Carriers (chemistry, therapeutic use, toxicity)
• Durapatite (chemistry, therapeutic use, toxicity)
• Glucose (chemistry, toxicity)
• Hep G2 Cells
• Humans
• Indocyanine Green (chemistry, radiation effects, therapeutic use, toxicity)
• Infrared Rays
• Male
• Mice, Inbred ICR
• Nanoparticles (chemistry, radiation effects, therapeutic use, toxicity)
• Neoplasms (drug therapy)
• Photochemotherapy
• Photosensitizing Agents (chemistry, radiation effects, therapeutic use, toxicity)
• Photothermal Therapy
• Mice